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用于檢測有害微生物的一次性光學(xué)測試基板 Uusitalo利用拉曼光譜技術(shù)開發(fā)了微生物細胞的光學(xué)檢測，通過SERS(表面增強拉曼散射)擴增提高了測量靈敏度。SERS測量基于拉曼散射，其強度通過金屬中自由電子的振蕩而增加。在一些情況下，該方法可以將樣品識別為一個分子的精確水平。使用卷對卷生產(chǎn)方法圖案化一次性SERS測量基板，并用薄金鍍層涂覆基板。 “在挑戰(zhàn)細胞測量的情況下，SERS過程變得越敏感，它在未來的應(yīng)用就越多。與傳統(tǒng)細胞培養(yǎng)相比，它將提供一種快速簡便的微生物鑒定方法，并能夠快速識別微生物。例如，食品中毒或食品工業(yè)生產(chǎn)設(shè)施中的快速純度分析，“Uusitalo說。 她認為，該方法的第一個一般應(yīng)用將是在爆炸物檢測或環(huán)境毒素的分析。便攜式拉曼測量裝置已經(jīng)在市場上用于分析更大的樣品量。未來的研發(fā)還將導(dǎo)致便攜式SERS測量設(shè)備用于測量非常低的樣品量。 關(guān)鍵詞： 光學(xué)測試
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最新評論
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dushunli 2019-08-20 00:13光學(xué)測試基本
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mang2004 2019-08-20 00:42原文：
VTT develops disposable optical test substrate for detecting harmful microbes
20/11/2017
Harmful microbes and toxic micromolecules in food and drinking water can cause serious health problems around the world. For her PhD thesis, VTT researcher Sanna Uusitalo has developed a disposable optical test substrate for use in microbial detection. The aim is to enable cost-effective detection of harmful microbes and toxins.
Uusitalo developed the optical detection of microbial cells using Raman spectroscopy to increase measurement sensitivity via SERS (surface-enhanced Raman scattering) amplification. SERS measurement is based on Raman scattering, whose intensity is increased by the oscillation of free electrons in metal. In some cases, the method can identify a sample to a precision-level of one molecule. The disposable SERS measuring substrate is patterned using a roll-to-roll production method and coating the substrate with thin gold plating.
"The more sensitive the SERS process becomes in the case of challenging cell measurements, the more applications it will have in the future. It would provide a fast and simple method of microbial identification compared to traditional cell cultures and enable the fast identification of microbes in cases, say, of food poisoning, or rapid purity analysis in food industry production facilities," says Uusitalo.
She believes that the first general application of the method will be in explosives detection or the analysis of environmental toxins. Portable Raman measuring devices are already on the market for the analysis of larger sample volumes. Future R&D will also lead to portable SERS measuring devices for measuring very low sample volumes.